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The function of arabinogalactan-proteins and pectin during cell expansion in A. thaliana

Reference	P08382
Principal Investigator / Supervisor	Professor Paul Knox
Co-Investigators / Co-Supervisors	Professor Philip Gilmartin
Institution	University of Leeds
Department	Plant Biochemistry & Biotechnology
Funding type	Research
Value (£)	185,103
Status	Completed
Type	Research Grant
Start date	01/01/1998
End date	03/11/2002
Duration	58 months

Abstract

We propose to elucidate the function of arabinogalactan-proteins (AGPs) and galactan-rich pectins during cell expansion in roots and hypocotyls of Arabidopsis thaliana seedings. Cell expansion can be blocked using AGP-binding Yariv reagents with a consequent disruption of deposition of galactan-rich pectin in expanding cell walls. Mutant seedlings insensitive and supersensitive to Yariv reagents have been isolated and will be characterised at the molecular and cell biological level. The target AGP bound by the Yariv reagent will be isolated and characterised. Events at the plasma membrane/cell wall interface involving the target AGP and the deposition of galactan-rich pectin will be studied using immunogold electron microscopy.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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