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Reorganisation of the actin cytoskeleton in plant cells: proteins that interact with the maize stimulus responsive actin regulating protein ZmADF3

Reference	P08362
Principal Investigator / Supervisor	Professor P Hussey
Co-Investigators / Co-Supervisors	Professor John Doonan
Institution	Royal Holloway, Univ of London
Department	Biological Sciences
Funding type	Research
Value (£)	194,286
Status	Completed
Type	Research Grant
Start date	20/02/1998
End date	20/02/2001
Duration	36 months

Abstract

As part of our goal in understanding the dynamics of the plant actin cytoskeleton we have identified a novel 35kDa phosphoprotein that copurifies with the maize actin depolymerising factor, ZmADF3; either with the ZmADF3 alone or with the ZmADF3:actin complex. We will use an immunoaffinity column procedure to isolate ZmADF3 complexes from maize suspension culture cell extracts. The 35kDa protein and corresponding cDNA will be isolated. In addition, we will use the sensitive two-hybrid system for identifying clones from a maize suspension culture cDNA library encoding proteins that specifically interact with ZmADF3. All identified proteins will be tested for functional activity in ZmADF3/G-actin binding assays, ZmADF3/F-actin disruption assays and, where appropriate, phosphorylation assays.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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