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Analysis of Ca signalling and Ca waves in the self-incompatability ( SI) response in Papaver rhoeas

Reference	P08335
Principal Investigator / Supervisor	Professor Vernonica E. (Noni) Franklin-Tong
Co-Investigators / Co-Supervisors
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	167,614
Status	Completed
Type	Research Grant
Start date	01/06/1998
End date	01/09/2001
Duration	39 months

Abstract

The Papaver pollen/SI system is becoming a classic model system for investigating second messenger signalling in intact plant cells. We recently described the first Ca2+ waves in plant cells. They may be initiated by increases in [Ca2+]i and [Ins(1,4,5)P3]i, and propagated by InsP3-induced Ca2+ -release (IICR) and modulate pollen tube growth. We have established that the self-incompatibility (SI) response is mediated by [Ca2+]i and recently obtained evidence for Ca2+ wave formation. We propose to establish the spatial and temporal dynamics of these Ca2+ waves in intact living pollen using Ca2+ -imaging. We aim to establish whether this involves extracellular and/or intracellular Ca2+ and to identify Ca2+ stores and Ca2+ channels involved. We will investigate the signalling pathways involved in [Ca2+]i modulation in the SI response and establish if there is a role for IICR and/or Ca2+ - induced Ca2+ -release (CICR).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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