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Investigations into the physiological function of cytochrome c`: a role in nitric oxide metabolism?

Reference	P08290
Principal Investigator / Supervisor	Professor James Moir
Co-Investigators / Co-Supervisors	Professor Robert K Poole
Institution	University of Sheffield
Department	Division of Clinical Sciences (S)
Funding type	Research
Value (£)	154,099
Status	Completed
Type	Research Grant
Start date	15/09/1997
End date	15/09/2000
Duration	36 months

Abstract

We aim to determine the physiological function of the widespread periplasmic cytochrome c', which we hypothesise to buffer NO in vivo. Insertional inactivation of the genes for cytochrome c' from Paracoccus denitrificans and Rhodobacter capsulatus will be followed by a detailed physiological analysis of the mutant phenotypes, with emphasis on the involvement of the cytochrome in NO metabolism. In addition to physiological analysis in batch and continuous liquid cultures, the toxicity of NO and O2 will be assessed on plates by disk diffusion susceptibility assays. Whole cell EPR spectroscopy will be used to examine cytochrome c' coordination state in vivo. Kinetic experiments are outlined which will examine whether cytochrome c' is a NO buffer and/or whether it reacts with O2. Also, genetic loci which confer resistance to NO will be isolated from random genomic libraries.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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