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The development of DNA-mediated transformation systems for Erysiphe spp

Reference	P08065
Principal Investigator / Supervisor	Professor Pietro Spanu
Co-Investigators / Co-Supervisors	Professor Sarah Gurr
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	154,373
Status	Completed
Type	Research Grant
Start date	07/11/1997
End date	07/11/2000
Duration	36 months

Abstract

Obligate biotrophic fungi are amongst the most important agricultural pathogens. Methods of genetic transformation are not available and this will severely limit the knowledge we may acquire on the biology and pathogenicity of these organisms. Here, we propose to develop a transformation system for Erysiphe spp. Transformation and selection will be carried out in planta, thus circumventing our inability to grow such fungi in vitro. Transformation vectors carrying antibiotic (hygromycin) or fungicide (benomyl) resistance genes will be delivered biolistically to developing haploid conidia. The conidia produced will be used to inoculate host plants under selective conditions. Stably transformed isolates will be recovered and analysed to assess the efficiency of transformation and the limitations posed by the technique.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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