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Organelle regulation of calcium waves and tip based calcium mobilisation in reorienting pollen tubes
Reference
P07752
Principal Investigator / Supervisor
Professor Tony Trewavas
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Cell and Molecular Biology
Funding type
Research
Value (£)
156,102
Status
Completed
Type
Research Grant
Start date
01/05/1997
End date
31/07/2000
Duration
38 months
Abstract
We have shown that pollen tube growth can be reoriented by internally-generated calcium waves or calcium mobilising mechanisms in the tip. To understand the critical contribution of organelles to these mechanisms requires imaging luminescence from targeted transgenic aequorin in single pollen tubes. To produce sufficient light for imaging we will resynthesise apoaequorin to eliminate codon bias, introduce MAR sequences and fuse apoaequorin with GFP to easily check on targeting and ease quantitation of calcium signals. New constructs will be made to target apoaequorin by transformation to the plasma membrane, ER, mitochondria, nucleus and cytoplasm and growing pollen tubes from these transformed plants will be used to detail the inter-relations of these cell compartments in the reorienting mechanisms.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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