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Transcriptional programme associated with the recognition and interactions of zoosporic plant pathogens with plant roots
Reference
P07720
Principal Investigator / Supervisor
Professor Neil Andrew Robert Gow
Co-Investigators /
Co-Supervisors
Institution
University of Aberdeen
Department
School of Medical Sciences
Funding type
Research
Value (£)
146,328
Status
Completed
Type
Research Grant
Start date
05/01/1998
End date
05/04/2001
Duration
39 months
Abstract
To use differential cDNA display to (i) define the ordered series of transcriptional events that accompany encystment and germ tube formation of zoospore-plant interactions; and (ii) to clone key genes that are regulated differentially in zoospores encysting in response to host and non-host roots and other stimuli; (iii) to construct reporter gene fusions and antisense genes to further examine temporal transcription during fungus-plant interactions. Reverse transcription-PCR (cDNA display) will be used to compare amplified cDNA copies of mRNAs extracted from encysting zoospore populations responding to roots and exudates of host and non-host plants and during auto-aggregation in vitro. Cloned 5' gene fragments will be used to isolate intact cDNA clones of the cognate genes for sequencing and fusion to reporter genes to follow in situ gene expression during plant-fungus interactions.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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