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A novel complex I homologue from Escherichia coli; a missing link in anaerobic respiration

Reference	P07717
Principal Investigator / Supervisor	Professor Simon C Andrews
Co-Investigators / Co-Supervisors	Professor Benjamin Berks, Professor J Guest
Institution	University of Reading
Department	Animal and Microbial Sciences
Funding type	Research
Value (£)	169,565
Status	Completed
Type	Research Grant
Start date	06/06/1997
End date	06/06/2000
Duration	36 months

Abstract

We have discovered an Escherichia coli operon (hyf) encoding a novel multi-subunit membrane-bound electron-transfer complex (Hyf) with strong sequence similarity both to hydrogenase-3 components of the formate hydrogen-lyase pathway and to mitochondrial NADH dehydrogenases (Complex 1). We seek to establish the role of this new complex, initially by testing our hypothesis that the enzyme functions as a proton-pumping formate hydrogen-lyase. We shall attempt to demonstrate that the HyfG protein is a fourth E. coli nickel-containing hydrogenase. Our aim is to perform sufficient biochemical characterisation of the enzyme to allow evaluation of the functional significance of the sequence similarities between Hyf and Complex 1. Regulation of hyf expression will be studied and the roles of hyf-encoded homologues of the formate-dependent transcriptional factor (Fh1A) and the formate channel (FocA) will be investigated.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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