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Comparative studies on the regulation of amino acid biosynthesis in streptomycetes

ReferenceP07394
Principal Investigator / Supervisor Professor S Baumberg
Co-Investigators /
Co-Supervisors
Institution University of Leeds
DepartmentHuman Biology
Funding typeResearch
Value (£) 174,308
StatusCompleted
TypeResearch Grant
Start date 01/11/1996
End date 01/11/1999
Duration36 months

Abstract

Amino acids (aa's) are frequent secondary metabolite precursors in streptomycetes; understanding the regulation of their metabolism is essential for manipulation of product yield. We have demonstrated in S. coelicolor physiological controls of branched chain aa biosynthesis, sequence data suggesting an attenuation mechanism. We propose: in this system (1) to test for attenuation; (2) to compare the physiology and genetics of regulation in S. coelicolor with other streptomycetes, notably S. fradiae whose physiological control differs; in appropriate systems (3) to starve for a required amino acid to check pathways where regulation was not found earlier; (4) to look for controls at the level of enzyme activity; and (5) to estimate aa pools.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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