BBSRC Portfolio Analyser
Award details
Physiological differentiation in submerged cultures of Streptomyces coelicolor
Reference
P07393
Principal Investigator / Supervisor
Professor Paul Dyson
Co-Investigators /
Co-Supervisors
Institution
Swansea University
Department
Biological Sciences
Funding type
Research
Value (£)
214,518
Status
Completed
Type
Research Grant
Start date
01/01/1997
End date
01/01/2000
Duration
36 months
Abstract
The physiological switches which allow for antibiotic production in Streptomyces are poorly defined. We will utilise an engineered lux reporter gene transposon Tn1794 to create a transposon library in S. coelicolor. The complete library will be characterised for light emission both physiological and morphological differentiation on solid media. The catalogued library will be a valuable resource for all Streptomyces researchers. A subset of the library, including all mutants affected in differentiation, will be comprehensively characterised for antibiotic production and light emission in submerged culture. We will then clone, sequence and investigate the regulation of expression of the tagged physiological control genes. Understanding the complex regulatory framework will permit genetic intervention to rationally increase antibiotic yields.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search