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Regulation of dicarboxylate oxidation in Rhizobium by overflow metabolism
Reference
P06888
Principal Investigator / Supervisor
Professor Philip Poole
Co-Investigators /
Co-Supervisors
Institution
University of Reading
Department
Animal and Microbial Sciences
Funding type
Research
Value (£)
186,134
Status
Completed
Type
Research Grant
Start date
01/01/1997
End date
01/01/2000
Duration
36 months
Abstract
The symbiotic form of Rhizobium leguminosarum oxidises C4-dicarboxylic acids, principally L-malate, to fuel nitrogen fixation. This oxidation occurs via a truncated TCA-cycle and is coupled to the excretion of amino acids, of which alanine is the principal one, and the synthesis of polyhydroxybutyrate both of which are overflow pathways. Energy input and redox balance in legume nodules are regulated by these processes. The objectives of this proposal are to 1. Clone and mutate the system for alanine excretion; 2. Examine the partitioning between amino acid excretion and polyhydroxybutyrate biosynthesis as a part of overflow metabolism, using well defined mutants; 3. Compare the effects of mutation of these pathways on bacteroid metabolism and nitrogen fixation.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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