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The role of cpxA in exoenzyme virulence determinant regulation in Erwinia carotovora
Reference
P06812
Principal Investigator / Supervisor
Professor George Salmond
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
176,791
Status
Completed
Type
Research Grant
Start date
01/02/1997
End date
01/05/2000
Duration
39 months
Abstract
Erwinia carotovora (Ecc) makes multiple exoenzyme virulence factors. A homologue of the cpxA gene of the human pathogens E. coli and Shigella sonnei is involved in control of virulence in Ecc. In E. coli and Shigella the CpxA protein is a transmembrane sensor kinase and cpxA mutants show diverse phenotypes, including effects on regulation of invasion and virulence in these animal pathogens. We will, 1) clone and sequence the wild type Ecc cpxA gene and analyse the genetic organisation of the Ecc cpxA locus; 2) overexpress the Cpx protein and determine its cellular location and topology; 3) perform a detailed genetic and physiological characterisation of this and newly-generated CpxA mutants; 4) study the factors involved in regulation of cpxA and the activity of its product; 5) assess the impact of CpxA/CpxR on exoenzyme gene transcription, and define other Cpx regulon genes.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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