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DIF1 - a gene required for meiotic development in Arabidopsis

Reference	P06696
Principal Investigator / Supervisor	Professor Hugh Dickinson
Co-Investigators / Co-Supervisors
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	201,084
Status	Completed
Type	Research Grant
Start date	01/01/1997
End date	31/05/2000
Duration	41 months

Abstract

The DIF1 gene encodes a polypeptide essential for meiotic development in Arabidopsis thaliana. Dif1 mutants are male and female sterile and exhibit chromosomal abnormalities during meiosis in anthers - indicating that the DIF1 product interacts directly or indirectly with meiotic chromosomes. DIF1 has been tagged with the Activator element and mapped to a 5kb fragment, currently being sequenced. To explore the role played by DIF1 in meiosis, a molecular and genetic analysis of six different dif1 mutant alleles will be carried out, and novel derivative alleles will be generated by site-selected PCR mutagenesis. The sequence and expression pattern of DIF1 and cytology of dif1 allelic variants will be used to investigate structure of the DIF1 protein and its function in meiosis. DIF1-GFP (Green Fluorescent Protein) reporter constructs will be used to study the expression of DIF1 at a cellular level in protoplasts and transgenic plants, while the subcellular location of the DIF1 polypeptide in meiocytes (and other cell types, if appropriate) will be established using immunocytochemistry.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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