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Metabolic engineering: redirection of carbon-flux at the level of pyruvate with particular reference to acetate accumulation
Reference
P06611
Principal Investigator / Supervisor
Dr Margaret Attwood
Co-Investigators /
Co-Supervisors
Professor J Guest
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
135,996
Status
Completed
Type
Research Grant
Start date
01/04/1997
End date
01/04/2000
Duration
36 months
Abstract
Aerobic chemostat growth of isogenic strains of Escherichia coli will be used to obtain a range of steady-state cultures. The carbon balance from substrate to various growth related products will be quantified. Selected steady-states will be subjected to specific perturbations and the carbon-fluxes recalculated. All the carbon-flux data will be used in metabolic control analysis to establish the role of PDH complex in carbon distribution, assess the possibility for controlled redirection of carbon-flux and the extent to which genes can be modified and still allow the cell to react to the environment.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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