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Calcium-dependent processes which control pollen tube reorientation

Reference	P06588
Principal Investigator / Supervisor	Professor Tony Trewavas
Co-Investigators / Co-Supervisors	Professor Mark Fricker, Mr Nick White
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	9,760
Status	Completed
Type	Research Grant
Start date	01/11/1996
End date	01/11/1998
Duration	24 months

Abstract

We have shown the pollen tube growth reorientation (which is necessary to transfer the sperm cells to the ovary) is regulated by changes in cytoplasmic calcium. To investigate how reorientation is controlled in living pollen tubes we will: (1) Identify the subcellular region of the tube which controls reorientation using a focused UV beam and loaded caged calcium. (2) Use fluorescence polarisation anisotropy to separately image free and bound fluorescently- tagged calmodulin and expressed fusion proteins between calmodulin and GFP during reorientation. (3) Investigate the calcium-regulated dynamics of putative P-particle movement during reorientation by imaging fluorescence resonance energy transfer between microinjected tagged myosin and calmodulin and in transformed plants between myosin and calmodulin fused to specific GFP variants.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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