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Exploitation of metabolic diversity of the genus Rhodococcus
Reference
P06520
Principal Investigator / Supervisor
Dr Christopher Oldfield
Co-Investigators /
Co-Supervisors
Dr Mansi El-Mansi
,
Professor Michael Goodfellow
,
Dr E Williams
Institution
Edinburgh Napier University
Department
Life Sciences
Funding type
Research
Value (£)
119,712
Status
Completed
Type
Research Grant
Start date
01/05/1997
End date
07/12/1999
Duration
31 months
Abstract
A genetically stable, high expression cloning system for use in Rhodococcus spp. will be developed for the purpose of exploiting the metabolic diversity inherent in this group of organisms, for biotechnological purposes. The vector will be constructed from a pUC-series plasmid by incorporation of a rhodococcal plasmid origin of replication and the lacz gene, under the control of a rhodococcal promoter. The expression of beta-galactosidase will permit the genetic stability of recombinant R. erythropolis to be characterised in shake-flask culture. This construct will serve as a model system for the regulated expression of rhodococcal enzymes with biotechnological application.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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