Award details

Purification of extremely halophilic proteins: development of enabling technology

Reference	P06330
Principal Investigator / Supervisor	Professor Julian B Chaudhuri
Co-Investigators / Co-Supervisors	Professor Michael Danson, Dr David Hough, Professor John Howell
Institution	University of Bath
Department	Chemical Engineering
Funding type	Research
Value (£)	185,931
Status	Completed
Type	Research Grant
Start date	01/06/1996
End date	01/06/1999
Duration	36 months

Abstract

The aim of this project is to evaluate methods for the large scale purification of enzymes from extreme halophiles. These enzymes are active at low water activities and therefore are of potential biotechnological importance. Our objectives are to develop methods to express cloned halobacterial genes in Escherichia coli, and in the halophile Haloferax volcanii, and to purify the expressed enzymes from both hosts to give an active product. We will work with the halophilic enzymes dihydrolipoamide dehydrogenase and citrate synthase for which we will have the genes cloned and sequenced. These enzymes will be used to develop generic methodologies which may then be applied to more commercially relevant targets.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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