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Molecular and cell biological characterisation of plant microtubule-associated proteins
Reference
P05852
Principal Investigator / Supervisor
Professor P Hussey
Co-Investigators /
Co-Supervisors
Institution
Royal Holloway, Univ of London
Department
Biological Sciences
Funding type
Research
Value (£)
144,750
Status
Completed
Type
Research Grant
Start date
16/09/1996
End date
16/09/1999
Duration
36 months
Abstract
Plant microtubules have recently been found to be surprisingly dynamic but poor understanding of associated proteins (MAPs) blocks progress. However, our two groups have: (i) obtained partial peptide sequence of the tobacco MAP65 and (ii) biochemically isolated 65, 100 and 120 kDa MAPs from carrot. This linked research group will isolate a cDNA clone of MAP65 and compare properties of E. coli-expressed protein against the four biochemically purified isoforms. One isoform is recognised by the mitotic phosphoprotein antibody MPM2: phosphorylation probably modifies MAP properties and phosphorylation shifts will be analysed around the cell cycle in synchronised cells. We will see if plant MAPs are substrates for plant kinases. Effects of MAPs on MT dynamics will be tested by microinjecting live cells. The carrot MAPs will be similarly studied.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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