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Quantitative studies of interpolymeric transglycosylation (IPT) of xyloglucans during plant cell growth
Reference
P05842
Principal Investigator / Supervisor
Professor Stephen Fry
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Cell and Molecular Biology
Funding type
Research
Value (£)
127,737
Status
Completed
Type
Research Grant
Start date
13/05/1996
End date
13/05/1999
Duration
36 months
Abstract
Xyloglucans are major structural polysaccharides of the expanding cell walls of higher plants. Xyloglucan endotransglycosylases (XETs) catalyse interpolymeric transglycosylation (IPT) in vitro, but IPT has not yet been detected or quantified in vivo, although it is at the centre of much current thinking about the mechanism and control of plant cell expansion and fruit ripening. This work aims to validate quantitatively a novel dual-labelling method (density/radioactivity) for assaying IPT in vitro, and then to monitor the effects of several biological effectors on the rate of IPT in vivo in cultured rose cells. We will then apply the methods to the detection of IPT in living plant organs in relation to growth regulation. An important objective of the work is to test the proposed role for the IPT in the integration of xyloglucan into the accreting cell wall.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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