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Molecular analysis of 6-phosphofructo-2-kinase/ fructose-26-bisphosphatase in higher plants
Reference
P05839
Principal Investigator / Supervisor
Dr Nicholas Kruger
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
155,893
Status
Completed
Type
Research Grant
Start date
21/10/1996
End date
20/10/1999
Duration
36 months
Abstract
This work aims to elucidate factors that influence the activities of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and, thus, regulate fructose 2,6-bisphosphate levels. A cDNA for the enzyme from loblolly pine will be used to isolate the corresponding full-length clone from spinach. The recombinant spinach protein will be used to determine the kinetic properties of the enzyme, and to examine the regulation of its component activities by reversible phosphorylation. The potential role for isoforms of the enzyme will be examined by analysis of the terminal coding region segments of clones isolated from different sources. This project will lead to a greater understanding of the control of fructose 2,6-bisphosphate levels and the regulation of plant carbohydrate metabolism.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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