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Gametic imprinting in the endosperm of Zea mays: the nature of the imprint, the mechanism by which it is established and its role in regulating gene expression
Reference
P05837
Principal Investigator / Supervisor
Professor Hugh Dickinson
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
184,185
Status
Completed
Type
Research Grant
Start date
01/04/1996
End date
01/10/1999
Duration
42 months
Abstract
A number of genes expressed during endosperm development in Zea mays are either gametically imprinted or regulated by imprinting. Investigation of the genomic organisation and methylation status of a range of such genes (cloned via a complex genetic screen in a previous study [AT43/546]) will provide information as to the nature of the imprint itself, while analysis of genomic DNA extracted from sperms, central cells and early triploid endosperm nuclei will reveal the developmental stage at which the methylation pattern characteristic of imprinting is established. Deletion series of genomic sequences will be used in transgenic studies to identify the imprinting boxes which direct the specific methylation/demethylation events, and northern and in situ hybridisation will be employed to determine whether gametic imprinting affects genes expressed at specific locations or at particular developmental stages.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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