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Heterotrophic nitrification: genetic characterisation and physiological role

Reference	P05783
Principal Investigator / Supervisor	Dr S Spiro
Co-Investigators / Co-Supervisors	Professor David Richardson
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	151,179
Status	Completed
Type	Research Grant
Start date	23/11/1996
End date	23/11/1999
Duration	36 months

Abstract

Heterotrophic nitrification (the oxidation of ammonia to nitrite during growth on an organic energy source) will be studied in the gram-negative bacterium Paracoccus denitrificans. The nucleotide sequences of the cloned nitrification genes will be determined. Chromosomal deletions will be constructed in each gene, to investigate its contribution to nitrification. The phenotype of nitrification mutants will be studied, in order to further understanding of the physiological role of the pathway. Reporter fusions to nitrification genes will be constructed, and their expression studied, thus identifying the growth conditions which optimise expression of heterotrophic nitrification.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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