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Identification of systemic acquired resistance mutants in Arabidopsis by luciferase/green fluorescent protein imaging

Reference	P05201
Principal Investigator / Supervisor	Professor Gary Loake
Co-Investigators / Co-Supervisors	Professor Nick Read
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	144,658
Status	Completed
Type	Research Grant
Start date	01/01/1996
End date	01/01/1999
Duration	36 months

Abstract

Following a local hypersensitive response, is the development, over several days, of resistance to secondary infections by a broad range of pathogens, a phenomenon termed systemic acquired resistance (SAR). The establishment of SAR correlates with the local and systemic expression of at least thirteen families of SAR genes. We propose to follow the establishment of SAR in Arabidopsis by imaging the natural bioluminescence/fluorescence of luciferase and green fluorescent protein (GFP) reporter genes, fused to the PR1a, SAR gene promoter. Furthermore, mutants in local and systemic acquired resistance will be identified by virtue of the pattern and intensity of bioluminescence/fluorescence established. The identified mutants will be characterised and placed into complementation groups. As a prerequisite for map based cloning, the position of the identified loci within the Arabidopsis genome will be determined using CAPS, RAPD and SSLP markers.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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