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An investigation of metabolic regulation of gene expression in plants using a new class of Arabidopsis thaliana mutant

ReferenceP05195
Principal Investigator / Supervisor Professor Ian Graham
Co-Investigators /
Co-Supervisors
Institution University of Glasgow
DepartmentIBLS Division of Biochemistry & Molecula
Funding typeResearch
Value (£) 162,896
StatusCompleted
TypeResearch Grant
Start date 01/05/1996
End date 01/05/1999
Duration36 months

Abstract

Photosynthesis associated genes are subject to carbon metabolite repression. I have isolated a number of Arabidopsis thaliana mutants that do not exhibit this response. Molecular analysis of these mutants will provide insight into the regulatory mechanism involved and will establish if other genes are subject to the same type of control. Biochemical analysis will establish what effect disruption of the control mechanism has on enzyme activities, metabolites and source-sink allocation. The phenotype will be studied both in vitro using protoplasts and in vivo using transgenic invertase plants and elevated CO2 conditions both of which result in increased soluble carbohydrates in the cell. This work will provide a wealth of information regarding the nature of metabolic regulation of gene expression in plant cells.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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