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Sense transgene-mediated silencing of viral endogenous nuclear genes in plants

Reference	P05171
Principal Investigator / Supervisor	Professor Sir David Baulcombe
Co-Investigators / Co-Supervisors	Dr Andrew Hamilton
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	161,661
Status	Completed
Type	Research Grant
Start date	29/01/1996
End date	29/01/1999
Duration	36 months

Abstract

The aim is to investigate the phenomenon of co-suppression. This is the post-transcriptional suppression of viral and/or plant gene expression that occurs when homologous sense transgenes are introduced into plant genomes. There are two sections to the proposed work. i) to understand why transcripts from suppressing transgenes are unstable even in the absence of a homologous sequence by analysing the structure of those RNAs. ii) the generation of viral mutants which can overcome suppression by homologous sense transgenes. The analysis of the sequence and biology of the mutants should give important information concerning the mechanism of sense gene suppression and features of a transcript which render it immune to silencing. Since the unintended suppression of gene expression is often undesirable in the biotechnological exploitation of transgenic plants, this information should allow the design of constructs which reliably overexpress a gene product.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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