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Coupling of water and sugar flows in plant storage tissues: use of magnetic resonance imaging and single-cell sampling

Reference	P05156
Principal Investigator / Supervisor	Professor Richard Bowtell
Co-Investigators / Co-Supervisors	Professor John Farrar, Professor Deri Tomos
Institution	University of Nottingham
Department	Sch of Physics & Astronomy
Funding type	Research
Value (£)	293,377
Status	Completed
Type	Research Grant
Start date	07/12/1995
End date	28/02/1999
Duration	39 months

Abstract

Water and sugar flows are coupled during the partitioning of photoassimilate for storage in and growth of plant organs. Solute and water may be differently coupled in phloem, xylem and parenchyma. We will measure the degree of coupling of water, sugar, malate and potassium flows in the developing taproot and leaf of carrot using novel non-invasive imaging techniques to address hypotheses otherwise currently untestable. Magnetic resonance imaging will be used to measure water flow and mobility (1H NMR) and concentration (1H NMR) and flow of organic solutes (13C pulse chase imaging). Single cell sampling will be used to measure vacuolar organic and inorganic solutes and cell water relations.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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