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Population genetics of phyllosphere-colonising Pseudomonas

ReferenceP05155
Principal Investigator / Supervisor Professor P Rainey
Co-Investigators /
Co-Supervisors
Institution University of Oxford
DepartmentPlant Sciences
Funding typeResearch
Value (£) 69,696
StatusCompleted
TypeResearch Grant
Start date 01/10/1995
End date 01/10/1997
Duration24 months

Abstract

The genetic structure of a population of phyllosphere-colonising Pseudomonas isolated from sugar beet is currently being investigated by multi-locus enzyme electrophoresis (MLEE). Statistical analysis of the association between loci shows that the population, which contains both pathogenic and saprophytic species, is in linkage disequilibrium, however, this does not preclude the occurrence of recombination which can only be determined empirically by DNA sequence analysis. Following completion of the MLEE study, I propose to investigate the extent of genetic recombination by sequencing four genes (aroE, gapA, oprF and rrn) from selected isolates. Sequence data will be subjected to detailed analysis including tests for mosaic gene structure and phylogenetic congruency.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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