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Molecular cloning of avirulence genes from the barley powdery mildew pathogen
Reference
P05146
Principal Investigator / Supervisor
Professor Jonathan Jones
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Sainsbury Laboratory
Funding type
Research
Value (£)
128,853
Status
Completed
Type
Research Grant
Start date
01/01/1996
End date
01/01/1999
Duration
36 months
Abstract
The aim is to clone avirulence genes from the barley mildew pathogen, Erysiphe graminis f. sp. hordei (Egh). A genetic map of a cross in which eight avirulence genes segregate will be constructed using amplified fragment length polymorphisms (AFLPs). Next, a high resolution AFLP map of the regions around the Avra131 and Avra132 loci will be made. Cosmid contigs covering these loci will be developed, using flanking AFLPs, and sub-cloned into plasmids. The presence of avirulence genes in these contigs will be tested, if possible by transforming isolates virulent on Mla13 barley to avirulence or, alternatively, by comparative analysis of cDNA libraries from barley infected with virulent or avirulent isolates.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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