Award details

Strain degeneration and initiation of the solventogenic fermentation in saccharolytic clostridia

Reference	P04480
Principal Investigator / Supervisor	Professor Michael Young
Co-Investigators / Co-Supervisors
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	231,041
Status	Completed
Type	Research Grant
Start date	01/10/1995
End date	30/04/1999
Duration	43 months

Abstract

The proposed research will determine whether Spo0A controls the onset of solventogenesis in saccharolytic clostridia by a direct or an indirect mechanism. The binding of B. subtilis and C. beijerinckii Spo0A to 0A boxes located in the upstream regions of clostridial genes whose expression is modulated at the onset of solventogenesis will be tested by gel retardation and footprinting experiments. This approach will be complemented by studying the expression of sol-lacZ gene fusions in B. subtilis in different genetic backgrounds. A possible role for AbrB (itself under spo0A regulation) in control of solventogenesis will also be explored. Modified forms of C. beijerinckii sol genes created in vitro and tested in B. subtilis will be used to replace the wild type alleles in the bacterial chromosome to test whether the deduced control mechanisms operate in vivo. The role of DNA elements that promote degeneration of solventogenesis in the wild type strain will be elucidated.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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