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Intracellular factors controlling exocytosis in plant cells
Reference
P03942
Principal Investigator / Supervisor
Professor Nicholas Battey
Co-Investigators /
Co-Supervisors
Professor Colin Brownlee
Institution
University of Reading
Department
Plant Sciences
Funding type
Research
Value (£)
87,862
Status
Completed
Type
Research Grant
Start date
01/04/1995
End date
31/07/1997
Duration
28 months
Abstract
This proposal is for some decisive experiments on the mechanism of exocytosis in plant cells. A key feature is the use of hypersecretory cells of the root cap which are actively secreting polysaccharide and therefore provide an important model for plants. The involvement of Ca2+ will be tested using an intracellular perfusion system and the role of annexins as Ca2+ signalling elements will be investigated using recombinant annexin p33 from a root cap cDNA library. In addition regulation by GTP-binding proteins will be tested using non- hydrolysable GTP analogues. The patch-clamp data will be compared with results from two complementary cell-free fusion assays we have recently developed.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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