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Regulation of touch-induced gene expression by nuclear calcium and nuclear calmodulin

Reference	P03941
Principal Investigator / Supervisor	Professor Tony Trewavas
Co-Investigators / Co-Supervisors	Professor Marc Knight
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	135,558
Status	Completed
Type	Research Grant
Start date	01/01/1996
End date	01/06/1999
Duration	41 months

Abstract

The role of changes in nuclear Ca2+ and nuclear calmodulin in the induction of calmodulin gene expression by mechanical signals will be investigated. The changes in Ca2+ will be characterised using tobacco containing nuclear targeted aequorin. Transformation with the calmodulin promoter linked to the green fluorescent protein (GFP) will permit non-invasive imaging of the cellular coincidence of Ca2+ change and calmodulin gene expression. Caged Ca2+ coupled to dextran with a nuclear localising signal (NLS) will be synthesised and photolysed to specifically elevate nuclear Ca2+ in the GFP plants. Finally nuclear calmodulin activity will be modified by transforming tobacco with constructs containing NLS attached to calmodulin and calmodulin inhibitory peptides attached to nuclear proteins.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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