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Kinetic measurements for understanding how brown rot fungi exploit the production of hydroxyl radicals

Reference	P03744
Principal Investigator / Supervisor	Dr Paul Wood
Co-Investigators / Co-Supervisors	Professor Michael Davies, Professor Bruce Gilbert
Institution	University of Bristol
Department	Biochemistry
Funding type	Research
Value (£)	84,127
Status	Completed
Type	Research Grant
Start date	01/10/1995
End date	01/10/1997
Duration	24 months

Abstract

Timber decay by brown rot fungi is notable for hydroxyl radical production at a distance from the hyphae. Cellobiose dehydrogenase from the brown rot Coniophora puteana will reduce Fe(III) to Fe(II). Partial autoxidation of this Fe(II) generates H2O2, permitting HO radical formation by the Fenton reaction. Rate constants will be measured for Fe(II)-oxalate complexes in reaction with O2 and H2O2, and for enzymic reduction of Fe(III). Techniques will include spin trapping by EPR. The aim is to understand how the HO radical flux and Fe(II) lifetime change as Fe(II), oxalate, O2 and H+ concentrations are varied, with a view to designing selective inhibitors.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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