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Structural basis of protein stability in the archaea

ReferenceP03655
Principal Investigator / Supervisor Professor Michael Danson
Co-Investigators /
Co-Supervisors
Dr David Hough, Professor Garry Taylor
Institution University of Bath
DepartmentBiology and Biochemistry
Funding typeResearch
Value (£) 315,480
StatusCompleted
TypeResearch Grant
Start date 31/01/1994
End date 31/07/1997
Duration42 months

Abstract

1) Cloning, sequencing and expression of citrate synthase genes from Archaea that span the phenotypic range of temperature and halophilicity. 2) Analysis of the 3D-structure of these enzymes by X-ray crystallography, molecular modelling and molecular dynamics simulation. 3) Prediction of structural features conferring extreme stability and testing of these predictions by site-specific mutagenesis.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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