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Function of the chloroplast NDH genes
Reference
P03109
Principal Investigator / Supervisor
Professor Peter Nixon
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
130,014
Status
Completed
Type
Research Grant
Start date
28/06/1994
End date
04/12/1997
Duration
42 months
Abstract
I. The ndh genes found in the tobacco chloroplast genome will be (1) expressed in Escherichia coli in order to raise antibodies which will be used to (2) detect the Ndh proteins in higher plant leaves, (3) identify their sub- cellular location, (4) determine at what stage of plastid development expression is maximal and (5) monitor the purification of the Ndh proteins to determine if the Ndh proteins co-purify as a protein complex that acts as a NADH: plastoquinone oxidoreductase. II. A mutagenesis strategy will be used in parallel to probe the function of the ndh genes. The ndh genes of the chloroplast of the green alga Chlamydomonas reinhardtii will be (1) identified, (2) sequenced and (3) their transcription pattern analysed. (4) Null mutants will be constructed through insertional mutagenesis and (5) effects on growth and chlororespiration analysed.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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