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The role of the pollen grain coating of Brassica oleracea in pollination and self-incompatibility

Reference	P02470
Principal Investigator / Supervisor	Professor Hugh Dickinson
Co-Investigators / Co-Supervisors
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	215,466
Status	Completed
Type	Research Grant
Start date	01/10/1994
End date	01/12/1997
Duration	38 months

Abstract

Using a new in vivo bioassay, components of the pollen coating of Brassica oleracea functional in self-incompatibility (SI), pollen hydration and in the stimulation of pollination-specific stigmatic responses will be identified and isolated. In a collaborative programme with Durham, polypeptides active in these processes will be N-terminal sequenced, and the data obtained used to obtain clones from a stage specific anther cDNA library. PCP7, a coating-held peptide which binds specifically to female products of the S(incompatibility)-locus (and therefore is a candidate for the male determinant of SI), will be expressed in vitro and its role in SI and pollination further explored in the bioassay and via other strategies. The molecular structure of PCP7 will be determined to identify the molecular domains involved in binding and perhaps S-haplotypic specificity.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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