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The role of a 3'-terminal negative-strand RNA binding protein in the replication of cucumber mosaic virus RNA
Reference
P02398
Principal Investigator / Supervisor
Professor Kenneth Buck
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
197,898
Status
Completed
Type
Research Grant
Start date
01/01/1995
End date
31/08/1998
Duration
44 months
Abstract
We have recently purified a tobacco protein (3' TNS RNA binding protein) that binds to the 3'-terminus of the negative-strand RNA of cucumber mosaic virus (CMV). A cDNA clone of the 3' TNSBP will be obtained, sequenced and expressed in bacterial and baculovirus systems. The RNA binding sites on the negative-strand of CMV RNAs 1, 2 and 3 will be defined. The ability of the 3'-TNS binding protein to interact with other proteins of the CMV replicase complex will be investigated. The effect of the 3'-TNS RNA binding protein on the production of positive and negative RNA strands by the CMV replicase in vitro will be determined.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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