BBSRC Portfolio Analyser
Award details
Analysis of homeodomain protein-protein interactions that regulate sexual development in the mushroom Coprinus
Reference
P02352
Principal Investigator / Supervisor
Dr L Casselton
Co-Investigators /
Co-Supervisors
Dr E Mellor
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
111,241
Status
Completed
Type
Research Grant
Start date
01/12/1994
End date
15/06/1998
Duration
43 months
Abstract
Sexual development in Coprinus is regulated by heterodimerisation of two classes of homeodomain proteins encoded by the A mating type genes. We have demonstrated heterodimerisation and homodimerisation of these proteins using an in vitro assay. We now wish to exploit this assay to:(1) identify the precise regions involved in protein-protein interactions, (2) ask whether heterodimerisation is the key to A mating type compatibility and whether the homodimers of one protein class play a role in regulating development, (3) use in vitro mutagenesis to identify the key amino acids which either enable or prevent heterodimerisation and (4)use epitope tags and immunofluorescence to look at subcellular localisation of the proteins during a compatible A mating.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search