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Brassica pollen cutinase: the synthesis, secretion and emission of the enzyme, and the nature and expression of the gene

Reference	P01756
Principal Investigator / Supervisor	Professor Hugh Dickinson
Co-Investigators / Co-Supervisors	Professor Sarah Gurr
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	131,028
Status	Completed
Type	Research Grant
Start date	01/05/1994
End date	01/05/1997
Duration	36 months

Abstract

A recently-identified Brassica napus cutinase, responsible for the penetration of stigmas by pollen tubes, will be investigated at the level of the polypeptide and the gene. Immunogold electron microscopy will be used to monitor the accumulation of the enzyme during grain development, its secretion into the intine layer of the wall and emission at the pollen tube tip during stigmatic penetration. Newly-acquired biochemical and molecular data will be used in the identification and cloning of the cutinase gene; in parallel PCR-based approaches we shall (a) extend promising work using primers based on fungal cutinase sequence data, and (b) use currently-available N- terminal sequence from the pollen cutinase itself to construct primers for amplification. Northern and in situ hybridisations will establish the timing and site of transcript accumulation, and study of genomic clones will reveal both the structure of the gene and identify upstream regulatory sequences directing pollen-specific expression.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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