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Analysis of transgenic tobacco plants with altered FBPase and SBPase activities

Reference	P01723
Principal Investigator / Supervisor	Professor Christine Raines
Co-Investigators / Co-Supervisors	Professor Richard Leegood, Dr Julie Lloyd
Institution	University of Essex
Department	Biological Sciences
Funding type	Research
Value (£)	223,686
Status	Completed
Type	Research Grant
Start date	15/08/1994
End date	01/01/1998
Duration	41 months

Abstract

This study sets out to investigate the effects of changing the levels of the photosynthetic carbon reduction (Calvin) cycle enzymes fructose- 1, 6-bisphosphatase (FBPase) and sedoheptulose-1, 7-disphosphatase (SBPase) on carbon dioxide fixation and biosynthesis of starch and sucrose. Mutant lines will be obtained by transforming tobacco plants with sense and antisense constructs prepared using cDNA clones encoding chloroplast FBPase and SBPase. The transgenic plants produced will be analysed for changes in protein levels by western blotting and enzyme assay techniques. Selected lines with altered levels of FBPase or SBPase will be examined biochemically and physiologically. Non-invasive techniques will be used to assess their photosynthetic performance in a range of environmental conditions and will allow us to calculate flux control coefficients. The impact of altered FBPase and SBPase activities on partitioning between starch and sucrose will be analysed in the transgenic plants.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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