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The importance of heterotrophic ferredoxin-NADP+-oxidoreductase in plant tissue during nitrate assimilation
Reference
P01717
Principal Investigator / Supervisor
Dr Caroline Bowsher
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
155,753
Status
Completed
Type
Research Grant
Start date
01/09/1994
End date
01/09/1997
Duration
36 months
Abstract
In higher plant roots Ferredoxin-NADP+-oxidoreductase (FNR) has an important regulatory role coordinating carbohydrate oxidation with the pathway of nitrogen assimilation. Previous studies have demonstrated that the enzymes from heterotrophic and autotrophic cells are the product of different genes and that differences in their expression would be expected to reflect their respective roles in different cell types. This research aims to determine the importance of heterotrophic FNR in controlling N assimilation in non- photosynthetic cells, by: (i) using antisense cDNAs (isolated from L. japonicus) to down-regulate heterotrophic FNR; (ii) generating transgenic lines that constitutively overproduce heterotrophic FNR; (iii) carrying out biochemical and molecular analysis of these transgenic lines focusing particularly on the impact that (i) and (ii) have on nitrate assimilation.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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