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Image and functional analyses of heterogeneity in the photosynthetic development of maize leaves at low temperatures

Reference	P01596
Principal Investigator / Supervisor	Professor Neil R Baker
Co-Investigators / Co-Supervisors	Professor Richard Cherry, Professor R Leech
Institution	University of Essex
Department	Biological Sciences
Funding type	Research
Value (£)	213,936
Status	Completed
Type	Research Grant
Start date	01/09/1994
End date	01/09/1997
Duration	36 months

Abstract

Two image analysis systems will be used to characterise the molecular and cellular heterogeneity of the photosynthetic apparatus in maize leaves when grown at low temperatures. The consequences for CO2 assimilation of chloroplast, cells, leaves and whole maize canopies in the field will be assessed. Specific aims are: (i) To use immunocytology to identify and quantify the spatial deployment of individual thylakoid proteins and their mRNAs. (ii) To use chlorophyll fluorescence image analysis to determine the effects of the heterogeneity of protein components on the transduction of light energy into CO2 assimilation. (iii) To determine the temperature thresholds for the onset of these heterogeneic effects and assessment of the variability between maize cultivars with different tolerances to low temperatures. (iv) To quantify the significance of heterogeneity of the photosynthetic apparatus to CO2 assimilation at the whole leaf and crop canopy level by using modifications of existing mechanistic mathematical models of photosynthesis.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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