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Award details
Biochemical, genetical and molecular characterisation of higher plant mutants defective in nitrate regulation
Reference
P01591
Principal Investigator / Supervisor
Dr J Wray
Co-Investigators /
Co-Supervisors
Institution
University of St Andrews
Department
Biology
Funding type
Research
Value (£)
133,654
Status
Completed
Type
Research Grant
Start date
01/05/1994
End date
01/05/1997
Duration
36 months
Abstract
We propose to characterise by biochemical, genetical and molecular approaches barley mutants defective in nitrate regulation of NR isolated at RES. Inheritance/alleleism testing will be performed between the selections to identify the nature/number of mutant loci. Mutant loci will be mapped. Representative lines will be characterised with respect to nitrate/light regulation of NR/NiR in leaf/root by immunoblot/northern analyses and 15N-nitrate transport. T-DNA mutagenised Arabidopsis lines will be screened for equivalent mutations by selecting for individuals with 'induced' levels of leaf NR in the absence of nitrate. These studies lay the foundations for the isolation/characterisation of regulatory genes controlling higher plant nitrate assimilation.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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