Award details

Modification of phosphoinositide signal transduction in transgenic plants

Reference	P01590
Principal Investigator / Supervisor	Professor Julie Gray
Co-Investigators / Co-Supervisors	Professor Alistair Hetherington
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	123,226
Status	Completed
Type	Research Grant
Start date	15/05/1994
End date	15/05/1997
Duration	36 months

Abstract

We propose to determine whether the PI signalling pathway is important in plant stimulus response coupling. A higher plant PtdIns (4.5) P2 phopholipase C, the key enzyme in this pathway, will be cloned and its expression manipulated. Antisense gene constructs will be used to reduce PLC activity in guard cells of transgenic plants. The contribution which this enzyme makes to signalling will be characterised by analysing the response of the transgenic guard cells to a number of stimuli including ABA, CO2 and light. This will involve making measurements of stomatal aperture and cytosolic [Ca2+]. These experiments may provide functional evidence to support the role of phosphoinositides ie plant signal transduction and also evidence as to which environmental stresses induce this pathway.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search