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Action of Phanerochaete chrysosporium on its natural substrate lignocellulose: gene expression and mechanistic studies
Reference
P01588
Principal Investigator / Supervisor
Dr Paul Sims
Co-Investigators /
Co-Supervisors
Professor Paul Broda
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
178,098
Status
Completed
Type
Research Grant
Start date
01/04/1994
End date
01/04/1997
Duration
36 months
Abstract
We aim to describe the synergistic lignocellulose degradation system of Phanerochaete chrysosporium ME446. We have developed generally-applicable PCR-based mRNA assays for gene expression. These will be applied to the classes of relevant genes under different growth conditions, including growth on solid substrates, to relate lignocellulose degradation to physiology and gene expression. Use of genetically defined derivatives of ME446 will also allow relation of performance to genotype. Purified carbohydrases, some novel in P. chrysosporium and some produced by heterologous gene expression, will be studied for substrate specificity and product stereochemistry.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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