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Differentiation of infection vesicles in the Colletotrichum-bean interaction and their role in race-cultivar-specificity
Reference
P01439
Principal Investigator / Supervisor
Dr J Green
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
128,491
Status
Completed
Type
Research Grant
Start date
01/10/1993
End date
01/06/1997
Duration
44 months
Abstract
Monoclonal antibodies (MAbs) will be used to identify cell surface molecules involved in race-cultivar recognition and biotrophic parasitism in the Colletotrichum-bean interaction. MAbs will be raised to infection vesicles (IVs) affinity-purified from infected leaves, using co-immunisation. MAbs recognising race-specific antigens, antigens unique to IVs and components of the interfacial matrix will be selected by immunofluorescence and immunogold labelling. To determine the role of these antigens in recognition and infection, MAbs will be used to perturb in vitro binding assays and injected into infected leaves to assess their effect on compatibility and/or incompatibility. Confocal microscopy will be used to monitor antigen expression.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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