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The isolation and characterisation of the genetic determinants of an avirulence phenotype which matches a gene for non-host resistance in pea
Reference
P01316
Principal Investigator / Supervisor
Dr A Vivian
Co-Investigators /
Co-Supervisors
Institution
University of the West of England
Department
Faculty of Health and Applied Sciences
Funding type
Research
Value (£)
117,377
Status
Completed
Type
Research Grant
Start date
03/01/1994
End date
27/10/1997
Duration
45 months
Abstract
Cloned DNA from race 4 of Pseudomonas syringae pv: phaseolicola has been shown to confer avirulence on P. syringae pv. pisi toward pea. The library cosmid, pPPY40 has been shown by transposon mutagenesis and sub-cloning to carry two regions of DNA, both of which are required for expression of the avirulence phenotype. The aims of this proposal are to (1) characterize the 2 regions of insert DNA responsible for the avirulence phenotype; (2) determine the number of genes involved and their relationship to one another; (3) investigate the regulation of the genes involved; (4) overexpress the avirulence proteins and search for elicitor of the HR. This will involve further transposon mutagenesis and sub-cloning; DNA sequence analysis, the use of reporter gene systems, to study control and overexpression to seek elicitor activity in planta.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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