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Genetic and physiological analysis of global regulatory systems involved in bacterial pathogenicity to plants

ReferenceP01211
Principal Investigator / Supervisor Professor Michael Daniels
Co-Investigators /
Co-Supervisors
Institution University of East Anglia
DepartmentSainsbury Laboratory
Funding typeResearch
Value (£) 138,325
StatusCompleted
TypeResearch Grant
Start date 01/04/1994
End date 01/04/1997
Duration36 months

Abstract

The bacterial plant pathogen Xanthomonas campestris produces extracellular enzymes (EE) and polysaccharide (EPS), synthesis of which is coordinately controlled by several sets of previously cloned regulatory genes. EE/EPS synthesis, and expression and function of regulator gene will be measured biochemically and by reporter gene fusions in both mutant and wild type genetic backgrounds to determine (a) how the regulatory genes interact to modulate EE/EPS levels, (b) whether they regulate each other's synthesis and (c) what signals they respond to. EE/EPS synthesis may be regulated by physiological factors such as starvation or accumulation of products. Chemostat cultures will be used to control precisely the physiological state of the bacteria.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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