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Transcriptional regulation of a PR protein gene by salicylic acid
Reference
P01134
Principal Investigator / Supervisor
Professor John Draper
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Medicine - Pathology
Funding type
Research
Value (£)
146,692
Status
Completed
Type
Research Grant
Start date
01/07/1993
End date
01/04/1997
Duration
45 months
Abstract
A gene AoPRTL coding for a monocot thaumatin like PR protein has been isolated that is massively and specifically upregulated by salicylic acid treatment of asparagus seedlings. We aim to generate a gene fusion with GUS and characterise the expression of the AoPRTL promoterin response to pathogen attack, wounding and salicylic acid treatment in transgenic plants. A deletion analysis, in vitro DNA gel shifts and in vivo DNA footprinting techniques will be used to determine which regions of the promoter are responsible for gene activation by (a) exogenously supplied salicylic acid and (b) pathogen infection/wounding. An attempt to clone transcription factors responsible for gene induction by exogenously supplied salicylic acid will be made.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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