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Supercharged nucleotide analogues: synthesis and biological applications

ReferenceMOL04558
Principal Investigator / Supervisor Professor Michael Blackburn
Co-Investigators /
Co-Supervisors
Institution University of Sheffield
DepartmentChemistry
Funding typeResearch
Value (£) 149,357
StatusCompleted
TypeResearch Grant
Start date 09/10/1995
End date 09/10/1998
Duration36 months

Abstract

Chemistry. We will make analogues of nucleotides having additional negative charge and stable to degradation by phosphatases. These will be based on the recently described methanetrisphosphonic acid and on its novel C-fluoro, C-hydroxy, and C-amino derivatives, to be synthesised in this work. Biochemistry. These analogues will be used to study reactions of nucleotide-using enzymes, especially ATP and dATP, and to generate transition state analogues of kinase processes, especially for adenylate kinase (EC 2.7.4.3) and phosphoglycerate kinase (EC 2.7.2.3).

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Molecules (Ropa) (MOL) [1994]
Funding SchemeX – not Funded via a specific Funding Scheme
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