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A confocal scanning laser microscopy facility for multiple labelling fluorescence and DIC application

Reference	JRI08148
Principal Investigator / Supervisor	Professor Malcolm Maden
Co-Investigators / Co-Supervisors	Professor Phillip Gordon-Weeks, Professor Gareth E Jones
Institution	King's College London
Department	Reproductive Health Endocrinology Dev
Funding type	Research
Value (£)	75,000
Status	Completed
Type	Research Grant
Start date	01/01/1997
End date	01/05/1997
Duration	4 months

Abstract

To assemble a confocal laser scanning microscope (CLSM) that is capable of examining four or five different specific features that are fluorescently or immunofluorescently labelled with high sensitivity, whilst simultaneously probing general morphology with between two and four different non-fluorescent modes. This instrument will be used to collect data from studies on the migration of neural crest cells, and in particular the role of retinoic acid and Hox genes; the growth cone cytoskeleton and its role in pathfinding; the expression patterns of dystroglycans and utrophin in muscle cell differentiation; the complex architectural changes that occur in the distribution of actin, myosin and associated proteins in newly formed skeletal muscle cells as the contractile filaments form and integrate into ordered sarcomeric assemblies; the patterning of the zebrafish CNS, including lineage analysis and fate mapping of the neural plate in vitro.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Research Initiative 1996 (JE1) [1996]
Funding Scheme	X – not Funded via a specific Funding Scheme

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